Alternative Names:

ATP dependent helicase CHD4; CHD4; Chromodomain helicase DNA binding protein 4; Mi 2 autoantigen 218 kDa protein; Mi 2b; Mi2 beta;

Application:

WB IP

Reactivity:

Human

Dilution:

WB 1:1000 IP

	Western blot detection of CHD4 in K562,HelaNE,HL-60,MOLT-4,Jurkat and 293 cell lysates using CHD4 mouse mAb (1:1000 diluted).Predicted band size:260KDa.Observed band size:260KDa.
	Immunoprecipitation analysis of K562 cell lysates using CHD4 mouse mAb.

Purification:

Affinity-chromatography

Specificity:

CHD4 Mouse mAb detects endogenous levels of total CHD4

Immunogen:

Purified recombinant human CHD4 protein fragments expressed in E.coli.

Description:

Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.