Alternative Names:

Cyclin; DNA polymerase delta auxiliary protein; PCNA;

Application:

WB IHC ICC/IF IP

Reactivity:

Human,Mouse,Monkey,Rat,Hamster

Dilution:

WB 1:1000 IHC 1:400 ICC/IF 1:100 IP

	Western blot detection of PCNA in Hela,3T3,COS7,C6 and CHO-K1 cell lysates using PCNA mouse mAb (1:1000 diluted).Predicted band size:36KDa.Observed band size:36KDa.
	Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma with PCNA mouse mAb (2E1-G10-H10,1:400 diluted),showing nuclear localization.A high pressure mediated antigen retrieval step was performed in citrate buffer(pH6.0).
	Immunofluorescent analysis of HeLa cells using PCNA mouse mAb (dilution 1:100).Fixed in 100% methanol for 2hr at -20℃.
	Immunoprecipitation analysis of Hela cell lysates using PCNA mouse mAb.

Purification:

Affinity-chromatography

Specificity:

PCNA Mouse mAb detects endogenous levels of total PCNA

Immunogen:

Purified recombinant human PCNA protein fragments expressed in E.coli.

Description:

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase,but not apurinic-apyrimidinic (AP) endonuclease,APEX2 activities.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.