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Details
Alternative Names:
Androgen receptor cofactor p44; C79984; MEP50; Methylosome protein 50; p44/Mep50; RGD1310479; RP11 552M11.3; WDR77;
Application:
WB ICC/IF
Reactivity:
Human,Mouse,Rat
Dilution:
WB 1:1000 ICC/IF 1:100
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Western blot detection of WDR77 in C6,3T3 and K562 cell lysates using WDR77 mouse mAb (1:1000 diluted).Predicted band size:42KDa.Observed band size:42KDa. |
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Immunofluorescent analysis of HeLa cells fixed in 1% Paraformaldehyde and then permeabilized in 0.1% Triton X-100,next using WDR77 mouse mAb (dilution 1:100). |
Purification:
Affinity-chromatography
Specificity:
WDR77 Mouse mAb detects endogenous levels of total WDR77
Immunogen:
Purified recombinant human WDR77 protein fragments expressed in E.coli.
Description:
Non-catalytic component of the 20S PRMT5-containing methyltransferase complex,which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones.This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles.Might play a role in transcription regulation.
Storage Condition and Buffer:
Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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