Alternative Names:

DNA mismatch repair protein Mlh1; MutL protein homolog 1; COCA2;

Application:

WB

Reactivity:

Human

Dilution:

WB 1:500

	Western blot detection of MLH1 in Hela,293T and Jurkat cell lysates using MLH1 mouse mAb (1:500 diluted).Predicted band size:85KDa.Observed band size:85KDa.

Purification:

Affinity-chromatography

Specificity:

MLH1 Mouse mAb detects endogenous levels of total MLH1

Immunogen:

Purified recombinant human MLH1 protein fragments expressed in E.coli.

Description:

Heterodimerizes with PMS2 to form MutL alpha,a component of the post-replicative DNA mismatch repair system (MMR).DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch,then MutL alpha is recruited to the heteroduplex.Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.