Alternative Names:

REPA2; RF-A; RP-A; RPA2; RPA32; replication factor-A protein 2;

Application:

WB ICC/IF IP

Reactivity:

Human

Dilution:

WB 1:2000 ICC/IF 1:200 IP

	Western blot detection of RPA32/RPA2 in MCF7,Hela and HUVEC cell lysates using RPA32/RPA2 mouse mAb (1:2000 diluted).Predicted band size:32KDa.Observed band size:32KDa.Exposure time:20s.
	Immunofluorescent analysis of HeLa cells fixed with -20℃ Methanol and using anti-RPA32/RPA2 antibody (dilution 1:200).
	Immunoprecipitation analysis of Hela cell lysates using RPA32/RPA2 mouse mAb.

Purification:

Affinity-chromatography

Specificity:

RPA32/RPA2 Mouse mAb detects endogenous levels of total RPA32/RPA2

Immunogen:

Purified recombinant human RPA32/RPA2 protein fragments expressed in E.coli.

Description:

Required for DNA recombination,repair and replication.The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage.Functions as component of the alternative replication protein A complex (aRPA).

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.