Alternative Names:

activator protein 1; AP-1; C-FOS; cellular oncogene c-fos; Cellular oncogene fos; FOS; G0/G1 switch regulatory protein 7; G0S7;

Application:

WB

Reactivity:

Human,Rat,Monkey

Dilution:

WB 1:500

	Western blot detection of c-Fos in Hela(TSA treated ) and Hela cell lysates using c-Fos mouse mAb (1:500 diluted).Predicted band size:62KDa.Observed band size:62KDa.

Purification:

Affinity-chromatography

Specificity:

c-Fos Mouse mAb detects endogenous levels of total c-Fos

Immunogen:

Purified recombinant human FOS protein fragments expressed in E.coli.

Description:

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor.In the heterodimer,FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites.On TGF-beta activation,forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.