Alternative Names:

CTC75; CTCBF; DNA repair protein XRCC6; G22P1; Ku 70; Ku autoantigen 70kDa; Ku autoantigen, 70kDa; Ku p70; Ku70; Kup70; TLAA; XRCC6;

Application:

WB ICC/IF

Reactivity:

Human

Dilution:

WB 1:1000 ICC/IF 1:200

	Western blot detection of Ku70 in Hela,A549,MCF7,Jurkat and Raji cell lysates using Ku70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.
	Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using Ku70 mouse mAb (dilution 1:200).

Purification:

Affinity-chromatography

Specificity:

Ku70 Mouse mAb detects endogenous levels of total Ku70

Immunogen:

Purified recombinant human Ku70 protein fragments expressed in E.coli.

Description:

Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.