Alternative Names:

BRAP; BRAP2; IMP; Galectin-2-binding protein; RING finger protein 52; RNF52; BRCA1 associated protein;

Application:

WB

Reactivity:

Human

Dilution:

WB 1:500-1000

	Western blot detection of BRAP in A549 and HUVEC cell lysates using BRAP mouse mAb (1:500 diluted).Predicted band size:67KDa.Observed band size:67KDa.

Purification:

Affinity-chromatography

Specificity:

BRAP Mouse mAb detects endogenous levels of total BRAP

Immunogen:

Purified recombinant human BRAP protein fragments expressed in E.coli.

Description:

Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.