Alternative Names:

UV excision repair protein RAD23 homolog B; hHR23B; p58; RAD23B;

Application:

WB IHC ICC/IF

Reactivity:

Human,Mouse,Rat,Monkey,Hamster

Dilution:

WB 1:1000 IHC 1:100 ICC/IF 1:100

	Western blot detection of hHR23b in A431,K562,Jurkat,C6,3T3 and Hela cell lysates using hHR23b mouse mAb (1:1000 diluted).Predicted band size:58KDa.Observed band size:58KDa.Exposure time:5min.
	Immunohistochemical analysis of paraffin-embedded Prostate Cancer using hHR23b mouse mAb (1/100 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
	Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using anti-hHR23b antibody (dilution 1:100).

Purification:

Affinity-chromatography

Specificity:

hHR23b Mouse mAb detects endogenous levels of total hHR23b

Immunogen:

Purified recombinant human hHR23b protein fragments expressed in E.coli.

Description:

Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum-associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.