Alternative Names:

APAF-3; ICE-LAP6; Apoptotic protease Mch-6; Apoptotic protease activating factor 3; CASP-9; Caspase-9 precursor; ICE-like apoptotic protease 6; MCH6; RNCASP9

Application:

WB

Reactivity:

Human,Transfected

Dilution:

WB 1:1000

	Western blot detection of Caspase-9 in A549,MCF7,Jurkat,K562 and Hela cell lysates using Caspase-9 mouse mAb (1:1000 diluted).Predicted band size:49/37KDa.Observed band size:49KDa.

Purification:

Affinity-chromatography

Specificity:

Caspase-9 Mouse mAb detects endogenous levels of total Caspase-9

Immunogen:

Purified recombinant human Caspase-9 protein fragments expressed in E.coli.

Description:

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.