Alternative Names:

c-Jun N-terminal kinase 3; FLJ12099; FLJ33785; JNK1; JNK2;JNK3; JNK3 alpha protein kinase; JNK3A; MAP kinase 10; MAP kinase p49 3F12; MAPK 10; MAPK10;

Application:

WB ICC/IF

Reactivity:

Human,Mouse,Rat

Dilution:

WB 1:1000 ICC/IF 1:100

	Western blot detection of JNK1 in C6 and 3T3 cell lysates using JNK1 mouse mAb (1:1000 diluted).Predicted band size:46,54KDa.Observed band size:46KDa.
	Immunofluorescent analysis of 3T3 cells fixed by anhydrous methanol for 2 h at -20℃ and using anti-JNK1 mouse mAb (dilution 1:100).

Purification:

Affinity-chromatography

Specificity:

JNK1 Mouse mAb detects endogenous levels of total JNK1

Immunogen:

Purified recombinant human JNK1 protein fragments expressed in E.coli.

Description:

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.