Alternative Names:

CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; G22P2; KARP1; Ku autoantigen, 80kDa; Ku80; Ku86 autoantigen related protein 1; KUB2; NFIV;

Application:

WB ICC/IF IP

Reactivity:

Human,Monkey

Dilution:

WB 1:1000 ICC/IF 1:400 IP 1:100

	Western blot detection of Ku80 in COS7,Jurkat,Hela,MCF7 and A549 cell lysates using Ku80 mouse mAb (1:1000 diluted). Predicted band size:86KDa. Observed band size:86KDa.
	Immunofluorescent analysis of Hela cells using Ku80 mouse mAb (1:400).
	Immunoprecipitation analysis of Hela cell lysates using Ku80 mouse mAb.

Purification:

Affinity-chromatography

Specificity:

Ku80 Mouse mAb detects endogenous levels of total Ku80

Immunogen:

Purified recombinant human Ku80 protein fragments expressed in E.coli

Description:

Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.