Alternative Names:

DNase IV; FEN1; Flap endonuclease 1; Flap structure specific endonuclease 1; hFEN-1; hFEN1; Maturation factor 1; MF1; Rad2;

Application:

WB ICC/IF

Reactivity:

Human,Mouse,Rat,Monkey,Hamster

Dilution:

WB 1:1000 ICC/IF 1:400

	Western blot detection of FEN-1 in Hela,Jurkat,3T3,COS7,PC-12,C6,Raw264.7,Raji and CHO-K1 cell lysates using FEN-1 mouse mAb (1:1000 diluted).Predicted band size:45KDa.Observed band size:45KDa.
	Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using FEN-1 mouse mAb (dilution 1:400).

Purification:

Affinity-chromatography

Specificity:

FEN-1 Mouse mAb detects endogenous levels of total FEN-1

Immunogen:

Purified recombinant human FEN-1 protein fragments expressed in E.coli.

Description:

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair.During DNA replication,cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment.It enters the flap from the 5'-end and then tracks to cleave the flap base,leaving a nick for ligation.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.