Alternative Names:

DBX; DDX3; HLP2; DDX14;

Application:

WB ICC/IF IP

Reactivity:

Human,Rat,Mouse,Monkey

Dilution:

WB 1:2000 ICC/IF 1:200 IP

	Western blot detection of DDX3 in Hela,3T3,C6,COS7,K562 and Jurkat cell lysate using DDX3 mouse mAb (1:1000 diluted).Predicted band size: 75KDa.Observed band size: 75KDa.
	Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using DDX3 mouse mAb (dilution 1:200).
	Immunoprecipitation analysis of Hela cell lysates using DDX3 mouse mAb.

Purification:

Affinity-chromatography

Specificity:

DDX3 Mouse mAb detects endogenous levels of total DDX3

Immunogen:

Purified recombinant human DDX3 protein fragments expressed in E.coli.

Description:

Multifunctional ATP-dependent RNA helicase.The ATPase activity can be stimulated by various ribo- and deoxynucleic acids indicative for a relaxed substrate specificity.In vitro can unwind partially double-stranded DNA with a preference for 5'-single-stranded DNA overhangs.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.