Alternative Names:

HSPD1; CPN60; GROEL; HLD4; HSP60; HSP65; HuCHA60; SPG13;

Application:

WB ICC/IF IP

Reactivity:

Human,Mouse,Rat

Dilution:

WB 1:1000 ICC/IF 1:100 IP 1:100

	Western blot detection of Hsp60 in Hela,Raji,Jurkat,C6,C2C12 and 3T3 cell lysates using Hsp60 mouse mAb (1:1000 diluted).Predicted band size:60KDa.Observed band size:60KDa.
	Immunofluorescent analysis of HeLa cells fixed with -20℃ Methanol and using Hsp60 mouse mAb (dilution 1:100).
	Immunoprecipitation analysis of Hela cell lysates using Hsp60 mouse mAb.

Purification:

Affinity-chromatography

Specificity:

Hsp60 Mouse mAb detects endogenous levels of total Hsp60

Immunogen:

Purified recombinant human Hsp60 protein fragments expressed in E.coli.

Description:

Implicated in mitochondrial protein import and macromolecular assembly.May facilitate the correct folding of imported proteins.May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.