Alternative Names:

DDIT1; GADD45;

Application:

WB

Reactivity:

recombinant protein

Dilution:

WB 1:1000

	Western blot detection of GADD45 α in CHO-K1 cell lysate（B）and CHO-K1 transfected by GADD45 α His fusion protein（A）cell lysate using GADD45 α mouse mAb (1:1000 diluted). Predicted band size:22KDa. Observed band size:22KDa.

Purification:

Affinity-chromatography

Specificity:

GADD45α Mouse mAb detects endogenous levels of total GADD45α

Immunogen:

Purified recombinant human GADD45 α protein expressed in E.coli

Description:

In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity (By similarity). Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.