Alternative Names:

E2F-1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; RBBP3; Retinoblastoma binding protein 3; Retinoblastoma-associated protein 1; Transcription factor E2F1;

Application:

WB ICC/IF IP

Reactivity:

Human,Rat

Dilution:

WB 1:1000 ICC/IF 1:100 IP

	Western blot detection of E2F-1 in C6,Raw264.7 and Hela cell lysates using E2F-1 mouse mAb (1:500 diluted).Predicted band size:70KDa.Observed band size:70KDa.
	Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-E2F-1 mouse mAb (dilution 1:100). DAPI was used to stain nucleus(blue).
	Immunoprecipitation analysis of Hela cell lysates using E2F-1 mouse mAb.

Purification:

Affinity-chromatography

Specificity:

E2F-1 Mouse mAb detects endogenous levels of total E2F-1

Immunogen:

Purified recombinant human E2F-1 protein fragments expressed in E.coli.

Description:

Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.