Alternative Names:

H2A histone family, member X;H2A.X;H2a/x;H2AFX;H2AX;H2AX histone;H2AX_HUMAN;Histone H2A.X;Histone H2AX

Application:

WB ICC/IF

Reactivity:

Human,Mouse

Dilution:

WB 1:500-2000 ICC/IF 1:400

	Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.
	Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in A549(upper,untreated or Hydroxyurea-treated) and Hela(lower,untreated or Hydroxyurea-treated) using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).

Purification:

Affinity-chromatography

Specificity:

Histone H2A.X(Phospho-Ser139) Mouse mAb detects endogenous levels of total Histone H2A.X(Phospho-Ser139)

Immunogen:

Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.

Description:

Play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.