Alternative Names:

CDKN1B; CDN1B; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27 KIP1; CDKN4; MEN4;

Application:

WB

Reactivity:

Mouse

Dilution:

WB 1:1000

	Western blot detection of p27 Kip1 in 3T3 cell lysates using p27 Kip1 mouse mAb (1:1000 diluted).Predicted band size:27KDa.Observed band size:27KDa.

Purification:

Affinity-chromatography

Specificity:

p27 KIP1 Mouse mAb detects endogenous levels of total p27 KIP1

Immunogen:

Purified recombinant human p27 Kip1 protein fragments expressed in E.coli.

Description:

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.