Alternative Names:

EIF-2; eIF-2-alpha; eIF-2A; eIF-2alpha; EIF2; EIF2A; EIF2S1; IF2A;

Application:

WB ICC/IF IP

Reactivity:

Human,Mouse,Rat

Dilution:

WB 1:1000 ICC/IF 1:200 IP

	Western blot detection of eIF2α in K562,COS7,3T3, C2C12,MCF-7,C6 and Hela cell lysates using eIF2α mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.
	Immunofluorescent analysis of Hela cells fixed by anhydrous methanol for 2 h at -20℃ and using anti-eIF2α mouse mAb (dilution 1:200).
	Immunoprecipitation analysis of Hela cell lysates using eIF2α.

Purification:

Affinity-chromatography

Specificity:

eIF2α Mouse mAb detects endogenous levels of total eIF2α

Immunogen:

Purified recombinant human eIF2α protein fragments expressed in E.coli.

Description:

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.