Alternative Names:

Retinoblastoma-binding protein 5; RBBP-5; Retinoblastoma-binding protein RBQ-3;

Application:

WB ICC/IF

Reactivity:

Human,Mouse,Rat

Dilution:

WB 1:1000 ICC/IF 1:100

	Western blot detection of RBBP5 in C2C12,THP-1,PC12 and 3T3 cell lysates using RBBP5 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa.
	Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-RBBP5 mouse mAb (dilution 1:100). DAPI was used to stain nucleus(blue).

Purification:

Affinity-chromatography

Specificity:

RBBP5 Mouse mAb detects endogenous levels of total RBBP5

Immunogen:

Purified recombinant human RBBP5 protein fragments expressed in E.coli.

Description:

As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.