Alternative Names:

CTRCT30; Epididymis luminal protein 113; HEL113; VIM; Vimentin.

Application:

WB ICC/IF IP

Reactivity:

Human

Dilution:

WB 1:1000 ICC/IF 1:800 IP

	Western blot detection of Vimentin in Molt-4,K562,COS7,Jurkat,Hela and Vimentin negative cell (Daudi,Ramos,Raji) lysates using Vimentin mouse mAb (1:1000 diluted).Predicted band size:57KDa.Observed band size:57KDa.
	Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-Vimentin mouse mAb (dilution 1:800).
	Immunoprecipitation analysis of Hela cell lysates using Vimentin mouse mAb.

Purification:

Affinity-chromatography

Specificity:

Vimentin Mouse mAb detects endogenous levels of total Vimentin

Immunogen:

Purified recombinant human Vimentin protein fragments expressed in E.coli.

Description:

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.