Alternative Names:

AIFM1; AIF; PDCD8; COXPD6

Application:

WB ICC/IF

Reactivity:

Human

Dilution:

WB 1:1000 ICC/IF 1:200

	Western blot analysis of extracts from Hela,Ramos,HepG2,MCF7 and Jurkat cell lysates using AIF mouse mAb (1:1000 diluted).Predicted band size:67KDa.Observed band size:67KDa.
	Immunofluorescent analysis of HeLa cells fixed with 4% Paraformaldehyde and using anti-AIF mouse mAb (dilution 1:200).

Purification:

Affinity-chromatography

Specificity:

AIF Mouse mAb detects endogenous levels of total AIF

Immunogen:

Purified recombinant human AIF protein fragments expressed in E.coli.

Description:

Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway.

Storage Condition and Buffer:

Mouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.