Alternative Names:

CDK4;CMM3;MGC14458;PSK-J3 ;

Application:

WB IHC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200

	Western blot analysis of extracts of various celllines, using CDK4 antibody.

Purification:

Affinity-chromatography

Specificity:

CDK4 Antibody detects endogenous levels of total CDK4

Immunogen:

A synthesized peptide derived from human CDK4

Description:

Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins. The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase .

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.