Alternative Names:

E2F-1; RBAP1; RBBP3; RBP3;

Application:

WB IHC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200

	Western blot analysis of extracts of variouscell lines, using E2F1 antibody.

Purification:

Affinity-chromatography

Specificity:

E2F1 Antibody detects endogenous levels of total E2F1

Immunogen:

A synthesized peptide derived from human E2F1

Description:

The E2F transcription factors are essential for regulation of the cell cycle. Physiological E2F is a heterodimer composed of an E2F subunit together with a DP subunit. Six members of the E2F family have been identified, and each E2F subunit has a DNA binding and a dimerization domain. E2F-1 to -5 activate transcription. E2F-1 to -3 bind pRb, and E2F-4 and -5 bind p107 or p130, and these interactions are under cell cycle control.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.