Alternative Names:

LC3; MAP1 light chain 3 like protein 1;MAP1 light chain 3 like protein 2; MAP1 light chain 3-like protein 2; MAP1A/1B light chain 3 A ;MAP1A/1B light chain 3 B;MAP1A/1BLC3; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; MAP1ALC3; MAP1B

Application:

WB,IHC,IF

Reactivity:

Human,Mouse,Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200 IF 1:100-1:500

	Western blot analysis of lc3A/B expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with Chloroquine.

Purification:

Affinity-chromatography

Specificity:

lc3A/B detects endogenous levels of total lc3A/B

Immunogen:

A synthesized peptide derived from human lc3A/B

Description:

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.