Alternative Names:

Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTA2, ACTSA, ACTVS, GIG46

Application:

WB IHC ICC/IF FC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:1000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:20

	Western blot analysis of alpha smooth muscle Actin expression in (1)A549 cell lysate; (2)C2C12 cell lysate using alpha-SMA antibody.
	Immunohistochemical analysis of paraffin-embedded human colon, using alpha smooth muscle Actin Antibody.
	Immunofluorescent analysis of A431 cells, using alpha smooth muscle Actin Antibody .

Purification:

Affinity-chromatography

Specificity:

alpha smooth muscle Actin Antibody detects endogenous levels of total alpha smooth muscle Actin

Immunogen:

A synthesized peptide derived from human alpha smooth muscle Actin

Description:

Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.