Alternative Names:

FERM, RhoGEF and pleckstrin domain protein 2, FGD1-related Cdc42-GEF, FIR, FRG, PLEKHC3

Application:

WB

Reactivity:

Human, Mouse,

Dilution:

WB 1:500-1:2000

	Western blot analysis on HuvEc cell lysate using FIR Antibody

Purification:

Affinity-chromatography

Specificity:

FIR Antibody detects endogenous levels of FIR

Immunogen:

A synthesized peptide derived from human FIR

Description:

Rho-guanine nucleotide exchange factor that activates RAC1. Plays a role in the response to class 3 semaphorins and remodeling of the actin cytoskeleton. Interacts with PLXNA1. Interaction with PLXNA1 or PIP5K1C lowers its guanine nucleotide exchange activity. Dissociates from PLXNA1 when SEMA3A binds to the receptor.Interacts with PIP5K1C via its FERM domain. The interaction with PIP5K1C is enhanced by SEMA3A binding. 2 isoforms of the human protein are produced by alternative splicing.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.