Alternative Names:

Homeobox transcription factor Nanog

Application:

WB

Reactivity:

Human, Mouse

Dilution:

WB 1:500-1:2000

	Western blot analysis of extracts from human Umbilical cord mesenchymal stem cell using Nano g Antibody

Purification:

Affinity-chromatography

Specificity:

The antibody detects endogenous level of total Nanog protein.

Immunogen:

Peptide sequence around aa.137~141 (K-Q-V-K-T) derived from Nano g

Description:

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes By similarity. Acts as a transcriptional activator or repressor By similarity. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' By similarity. When overexpressed, promotes cells to enter into S phase and proliferation

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.