Alternative Names:

PKM; PK3; OIP3; PK2;

Application:

WB

Reactivity:

Human

Dilution:

WB 1:500-1:2000

	Western blot analysis of extracts from HepG2 and 293 cells using PKM1/2

Purification:

Affinity-chromatography

Specificity:

The antibody detects endogenous levels of total PKM1/2 protein.

Immunogen:

Peptide sequence around aa. 36~40(D-S-P-P-I) derived from Human PKM1/2.

Description:

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.