Alternative Names:

CSDD2 ; FLJ16517;

Application:

WB

Reactivity:

Human

Dilution:

WB 1:500-1:1000

	Western blot analysis of extracts from Hela and 293 cells using LIN28B Antibody

Purification:

Affinity-chromatography

Specificity:

The antibody detects endogenous levels of total LIN28B protein.

Immunogen:

Peptide sequence around aa.242~246( P-S-V-Q-K) derived from Human LIN28B

Description:

Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.