Alternative Names:

HIF1; MOP1; PASD8; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF1A

Application:

WB IHC ICC/IF IP FC

Reactivity:

Human

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:30 FC 1:30

	Western blot analysis of HIF-1 alpha expression in Ramos cell lysate.
	Immunohistochemical analysis of paraffin-embedded human kindey, using HIF-1 alpha Antibody.
	Immunofluorescent analysis of Hela cells, using HIF-1 alpha Antibody.

Purification:

Affinity-chromatography

Specificity:

HIF-1 alpha Antibody detects endogenous levels of total HIF-1 alpha

Immunogen:

A synthesized peptide derived from human HIF-1 alpha

Description:

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.