Alternative Names:

HSF1; HSTF 1; HSTF1; Heat shock factor protein 1; Heat shock transcription factor 1;

Application:

WB IHC ICC/IF IP FC

Reactivity:

Human

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 IP 1:50 FC 1:50

	Western blot analysis of Phospho-HSF1 (S326) expression in HeLa cell lysates treated with heat.

Purification:

Affinity-chromatography

Specificity:

Phospho-HSF1 (S326) Antibody detects endogenous levels of total Phospho-HSF1 (S326)

Immunogen:

A synthesized peptide derived from human Phospho-HSF1 (S326)

Description:

Heat shock transcription factors (HSF, also designated HSTF) 1 and 2 are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development, and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.