Alternative Names:

E2AK2;E2AK2; EIF2AK1; EIF2AK2; MGC126524; PKR p68 kinase; PKR; PRKR;

Application:

WB IHC IP ChIP

Reactivity:

Human

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 IP 1:50

	Western blot analysis of PKR phosphorylation expression in HeLa cell lysate treated with Calyculin A and TNF-alpha.
	Immunohistochemical analysis of paraffin-embedded human colon, using Phospho-PKR (T446) Antibody.

Purification:

Affinity-chromatography

Specificity:

Phospho-PKR (T446) Antibody detects endogenous levels of total Phospho-PKR (T446)

Immunogen:

A synthesized peptide derived from human Phospho-PKR (T446)

Description:

PKR a protein kinase of the PEK family. Upon binding double-stranded RNA, it becomes autophosphorylated and activated. Phosphorylates and inhibits the alpha subunit of eIF2 alpha, which leads to an inhibition of the initiation of protein synthesis.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.