Alternative Names:

H4F2; HIST; HIST1H4A; Histone H4.AC-H4K5;H4K5;

Application:

WB IHC ICC/IF IP

Reactivity:

Human Mouse Rat

Dilution:

WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

	Western blot analysis of Histone H4 (acetyl K5) expression in TSA treated HeLa cell lysate.
	Immunohistochemical analysis of paraffin-embedded human colon, using Histone H4 (acetyl K5) Antibody.

Purification:

Affinity-chromatography

Specificity:

Histone H4 (acetyl K5) Antibody detects endogenous levels of total Histone H4 (acetyl K5)

Immunogen:

A synthesized peptide derived from human Histone H4 (acetyl K5)

Description:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.