Alternative Names:

B-RAF; p94; NS7; B-Raf proto-oncogene serine/threonine-protein kinase;BRAF;BRAF1;

Application:

WB IHC IP FC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 IP 1:50 FC 1:50

	Western blot analysis of B Raf expression in (1)Human brain lysate (2)HepG2 cell lysate.
	Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using B Raf Antibody.

Purification:

Affinity-chromatography

Specificity:

B Raf Antibody detects endogenous levels of total B Raf

Immunogen:

A synthesized peptide derived from human B Raf

Description:

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.