Alternative Names:

CDKN1B; CDN1B; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27 KIP1; CDKN4; MEN4;

Application:

WB IHC ICC/IF IP FC

Reactivity:

Human Rat

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50

	Western blot analysis of p27 KIP 1 expression in MCF-7 cell lysate.
	Immunohistochemical analysis of paraffin-embedded human colon, using p27 KIP 1 Antibody.
	Immunofluorescent analysis of Hela cells, using p27 KIP 1 Antibody .

Purification:

Affinity-chromatography

Specificity:

p27 KIP 1 Antibody detects endogenous levels of total p27 KIP 1

Immunogen:

A synthesized peptide derived from human p27 KIP 1

Description:

The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.