Alternative Names:

CBP; eIF-4E; eIF-4F 25 kDa subunit; EIF4F; EIF4E1; EIF4EL1; MGC111573; EIF4E;

Application:

WB IHC ICC/IF IP

Reactivity:

Human Mouse Rat

Dilution:

WB 1:1000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

	Western blot analysis of eIF4E (phosphoS209) expression in (1) HEK293 cell lysate; (2) Mouse spleen lysate.
	Immunohistochemical analysis of paraffin-embedded mouse kidney, using Phospho-eIF4E (S209) antibody.

Purification:

Affinity-chromatography

Specificity:

Phospho-eIF4E (S209) antibody detects endogenous levels of total Phospho-eIF4E (S209)

Immunogen:

A synthesized peptide derived from human Phospho-eIF4E (S209)

Description:

eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.