Alternative Names:

Brd4; CAP; HUNK1; MCAP; Bromodomain containing 4; chromosome associated protein;

Application:

WB IHC ICC/IF IP

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

	Western blot analysis of Brd4 expression in HeLa cell lysate.
	Immunohistochemical analysis of paraffin-embedded human kidney, using Brd4 Antibody.
	Immunofluorescent analysis of Hela cells, using Brd4 Antibody .

Purification:

Affinity-chromatography

Specificity:

Brd4 Antibody detects endogenous levels of total Brd4

Immunogen:

A synthesized peptide derived from human Brd4

Description:

Bromodomain-containing protein 4 (BRD4) is a member of the bromodomains and extra terminal (BET) family of proteins, which also includes BRD2, BRD3, and BRDT. BET family proteins contain two tandem bromodomains and an extra terminal (ET) domain, and bind acetyl lysine residues. BRD4 is a chromatin-binding protein with a preference for Lys14 on histone H3 as well as Lys5 and Lys12 on histone H4. BRD4 chromatin binding occurs throughout the cell cycle, including condensed mitotic chromosomes, when the majority of genes are silenced.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.