Alternative Names:

H3 histone; HIST1H3A; Histone cluster 1, H3a;

Application:

WB ICC/IF

Reactivity:

Human Mouse

Dilution:

WB 1:500~1:2000 ICC/IF 1:50~1:200

	Western blot analysis of Histone H3 (mono methyl K36) expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.

Purification:

Affinity-chromatography

Specificity:

Histone H3 (mono methyl K36) Antibody detects endogenous levels of total Histone H3 (mono methyl K36)

Immunogen:

A synthesized peptide derived from human Histone H3 (mono methyl K36)

Description:

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.