Alternative Names:

H3/j; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/I; HistoneH3/j; Histone H3/k; Histone H3/l; HIST3H3;

Application:

WB IHC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:1000 IHC 1:1000~1:2000

	Western blot analysis of Histone H3 (mono+di+tri methyl K79) expression in (1) NIH/3T3 cell lysate; (2) A549 cell lysate.
	Immunohistochemical analysis of paraffin-embedded rat colon, using Histone H3 (mono+di+tri methyl K79) Antibody.

Purification:

Affinity-chromatography

Specificity:

Histone H3 (mono+di+tri methyl K79) Antibody detects endogenous levels of total Histone H3 (mono+di+tri methyl K79)

Immunogen:

A synthesized peptide derived from human Histone H3 (mono+di+tri methyl K79)

Description:

H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.