Alternative Names:

CDC28; CDC2A; CDK1; Cell division control protein 2 homolog; Cyclin-dependent kinase 1; MPF; kinase Cdc2; p34 protein kinase;

Application:

WB IHC IP

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 IP 1:50

	Western blot analysis of Phospho-Cdk1/2 (T14) expression in HeLa cell lysate treated with hydroxyurea.
	Immunohistochemical analysis of paraffin-embedded rat spleen, using Phospho-Cdk1/2 (T14) Antibody.

Purification:

Affinity-chromatography

Specificity:

Phospho-Cdk1/2 (T14) Antibody detects endogenous levels of total Phospho-Cdk1/2 (T14)

Immunogen:

A synthesized peptide derived from human Phospho-Cdk1/2 (T14)

Description:

Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk2 is closely related to cdc2 (cdk1) which has proved useful as a marker of proliferation. Cdk1 and Cdk2 are a catalytic subunits of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.