Alternative Names:

FBL;FIB;FLRN;RNU3IP1;

Application:

WB IHC ICC/IF IP FC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 IHC 1:50~1:100 ICC/IF 1:50~1:100 IP : 1:30 FC 1:50

	Western blot analysis of Fibrillarin expression in HepG2 cell lysate.
	Immunohistochemical analysis of paraffin-embedded human testis, using Fibrillarin Antibody.
	Immunofluorescent analysis of Hela cells, using Fibrillarin Antibody .

Purification:

Affinity-chromatography

Specificity:

Fibrillarin Antibody detects endogenous levels of total Fibrillarin

Immunogen:

A synthesized peptide derived from human Fibrillarin

Description:

Fibrillarin is a 2'-O-methyltransferase located in fibrillar regions and Cajal bodies of the nucleolus, where RNA transcription and pre-RNA processing take place. Fibrillarin associates with several other structual proteins as well as box C/D snoRNA to form a complex that functions in pre-rRNA processing, pre-rRNA methylation and ribosome assembly. This complex catalyzes site-specific 2'-O-ribose methylation of targeted nucleotides within the rRNA sequence. The sequence, structure and function of fibrillarin are highly conserved and fibrillarin gene expression is essential for early embryonic development.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.