Alternative Names:

ATF2, Activating 2, CREB2, CREBP1, Cyclic-AMP-dependent ATF-2, HB16, MXBP protein, cAMP response element binding protein CRE-BP1;

Application:

WB ICC/IF IP

Reactivity:

Human

Dilution:

WB 1:500~1:2000 ICC/IF 1:50~1:200 IP 1:30

	Western blot analysis of Phospho-ATF2 (T71) expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with Anisomycin.

Purification:

Affinity-chromatography

Specificity:

Phospho-ATF2 (T71) Antibody detects endogenous levels of Phospho-ATF2 (T71)

Immunogen:

A synthesized peptide derived from human Phospho-ATF2 (T71)

Description:

The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.