Alternative Names:

Ataxia telangiectasia mutated homolog; Ataxia telangiectasia mutated; kinase ATM

Application:

WB IHC

Reactivity:

Human

Dilution:

WB 1:500-1:1000 IHC 1:50-1:200

	Western blot analysis of extracts from Hela cells untreated or treated with UV using ATM(Phospho-Ser1981) Antibody

Purification:

Affinity-chromatography

Specificity:

The antibody detects endogenous level of ATM only when phosphorylated at serine 1981.

Immunogen:

Peptide sequence around phosphorylation site of serine 1981 (E-G-S(p)-Q-S) derived from Human ATM.

Description:

ATM encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.