Alternative Names:

BRCA1; Breast cancer type 1 susceptibility protein; familial breast/ovarian cancer gene 1

Application:

WB

Reactivity:

Human,

Dilution:

WB 1:500-1:2000

	Western blot analysis on 293 cell lysate using Phospho-BRCA1(Ser1457) Antibody

Purification:

Affinity-chromatography

Specificity:

Phospho-BRCA1(Ser1457) Antibody detects endogenous levels of BRCA1 only when phosphorylated at Sersine 1457

Immunogen:

A synthesized peptide derived from human BRCA1 around the phosphorylation site of Sersine 1457

Description:

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC for BRCA1-associated genome surveillance complex. This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full-length natures identified.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.