Alternative Names:

Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; Caspase-8 precursor; EC 3.4.22.-; EC 3.4.22.61; FADD-homologous ICE/CED-3- like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; ICE8; MACH; MCH5; MORT1-associated CED-3 homolog

Application:

WB IHC

Reactivity:

Human, Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200

	Western blot analysis of Caspase 8 phosphorylation expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

Phospho-Caspase 8 (Ser347) Antibody detects endogenous levels of Caspase 8 only when phosphorylated at Serine 347

Immunogen:

A synthesized peptide derived from human Caspase 8 around the phosphorylation site of Serine 347

Description:

This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.