Alternative Names:

Breast carcinoma-associated antigen DF3, CD227, Carcinoma-associated mucin, EMA, Episialin, H23AG, MUC-1, MUC1, Mucin 1 precursor, PEM, PEMT, PUM, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, T

Application:

WB IHC IF/ICC

Reactivity:

Human,

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500

	Western blot analysis on HepG2 cell lysate using Phospho-CD227/MUC1(Tyr1229) Antibody

Purification:

Affinity-chromatography

Specificity:

Phospho-CD227/MUC1(Tyr1229) Antibody detects endogenous levels of CD227 and MUC1 only when phosphorylated at Tyrosine 1229

Immunogen:

A synthesized peptide derived from human CD227/MUC1 around the phosphorylation site of Tyrosine 1229

Description:

This gene is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. This protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. The protein serves a protective function, binding to pathogens, as well as functioning in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.