Alternative Names:

CDC28, CDC2A, CDK1, Cell division control protein 2 homolog, Cyclin-dependent kinase 1, EC 2.7.11.22, EC 2.7.11.23, MPF, kinase Cdc2, p34 protein kinase

Application:

WB IHC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:1000 IHC 1:50-1:200

	Western blot analysis of extracts from HeLa cells, using CDC2(Phospho-Thr161) Antibody.

Purification:

Affinity-chromatography

Specificity:

CDC2(Phospho-Thr161) Antibody detects endogenous levels of CDC2 only when phosphorylated at Threonine 161.

Immunogen:

The antiserum was produced against synthesized phosphopeptide derived from human CDC2 around the phosphorylation site of Threonine161

Description:

Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.