Alternative Names:

CDC25M3, Dual specificity phosphatase Cdc25A, EC 3.1.3.48, M-phase inducer phosphatase 1, MPIP1

Application:

WB IHC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200

	Western blot analysis on HeLa cell lysate using Phospho-CDC25A(Ser75) Antibody

Purification:

Affinity-chromatography

Specificity:

Phospho-CDC25A(Ser75) Antibody detects endogenous levels of CDC25A only when phosphorylated at Sersine 75

Immunogen:

A synthesized peptide derived from human CDC25A around the phosphorylation site of Sersine 75

Description:

CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.